(1) Calibrate the pH electrode and dissolved oxygen electrode.
(2) Tank sterilization. According to the needs of the medium into the tank, according to requirements after sealing, small fermentation tank (5L) can be placed in a large sterilization pot sterilization (115 °C, 30 minutes) large fermentation tank (10L and above) Steam sterilization (121 °C, 30 min).
(3) After the tank is cooled, place it on the fermentation bench and install it well; open the cooling water, open the pump power supply, connect the ventilation pipe to start ventilation, adjust the air intake knob to make proper ventilation, and keep the tank pressure at 0.05MPa. Set the temperature, pH, stirring speed, etc., and calibrate the slope of the dissolved oxygen electrode at the determined rotation speed and ventilation volume. The value is 100%.
(4) After the temperature is stable and all the parameters are correct, the pre-rotated seeds are inserted, the fermentation timing starts, and various parameters are recorded.
(5) Wash the tank body and electrode after fermentation, insert the pH electrode into the 3M potassium chloride flask, and use the protective cover for the probe of the oxygen electrode.